Knockout inducible rnai systems user manual

Metastatic renal cell carcinoma (mRCC) is nearly incurable and accounts for most of the mortality associated with RCC. Von Hippel Lindau (VHL) is a tumour suppressor that is lost in the majority of clear cell RCC (ccRCC) cases. Its role in regulating hypoxia-inducible factors-1α (HIF-1α) and -2α (HI . vii Kit Contents and Storage Types of Kits This manual is supplied with the products listed below. Note: The BLOCK-iT ™ Inducible H1 Lentiviral RNAi System also contains the BLOCK-iT Inducible H1 Lentiviral RNAi System components and the BLOCK-iT™ Inducible H1 Lentiviral RNAi System manual. Product Catalog no. Here, we show that inducible RNAi-mediated knockdown of syndecan-1 in human MM cells leads to reduced growth rates and a strong increase of apoptosis. Importantly, knockdown of EXT1, a copolymerase critical for HS chain biosynthesis, had similar effects. Using an innovative myeloma xenotransplantation model in Rag-2 (-/-)gamma (c) (-/-) mice.

Try Functional CRISPR or RNAi Screening. Targeted or Genome-Wide. Do-it-Yourself or Full-Service options available. Tech Note CRISPR/RNAi Screening Service. InDOXible™. Tet-Activated cDNA Lentiviral Single- and Two-Vector Expression System for convenient doxycycline-inducible cDNA expression AND. Tet-Inducible CRISPR/Cas9 Lentiviral Vector. Using this system, two complementary RNA strands are synthesized from DNA templates supplied by the user. The resulting RNA strands are annealed after the transcription reaction to form dsRNA or siRNA. In the case of dsRNA, any remaining single-stranded RNA and DNA template are removed with a nuclease digestion step following annealing. Clontech Laboratories, Inc. www.doorway.ru Protocol No. PT A Takara Bio Company Version No. PR 3 Mir-X™ Inducible miRNA Systems User Manual A. Summary The Mir-X Inducible miRNA Systems (Red Green) employ elements of our tightly regulated and highly respon- sive Tet-On® Advanced Expression System to produce on-demand and high-level expression of miRNAs (Clontech-.

User Manual Knockout™ Inducible RNAi Systems User Manual United States/Canada Asia Pacific + Europe +(0) Japan +(0) Clontech Laboratories, Inc. A Takara Bio Company Terra Bella Ave. Mountain View, CA Technical Support (US) E-mail: [email protected] [email protected]. anneal complementary shRNA oligonucleotides using the protocols in the Knockout RNAi Systems User Manual (PT). The annealed oligonucleotide should contain 5'-Bam. H I and 3'-Eco. R I overhangs. Then ligate the annealed oligonucleotide into RNAi-Ready pSIREN-RetroQ-ZsGreen. The use of RNA interference (RNAi) has enabled loss-of-function studies in mammalian cancer cells and has hence become critical for identifying and validating cancer drug targets. Current transient siRNA and stable shRNA systems, however, have limited utility in accurately assessing the cancer dependency due to their short-lived effects and.